EtEraser™ HP Endotoxin Removal Kit

EtEraser™ SE Endotoxin Removal Kit

1. Zambiri zamalonda

Ndife akatswiri pakuyezetsa endotoxin ndi endotoxinremoval.Timapereka mayankho athunthu okhudza mitundu yonse ya endotoxincontrol, kuwunika kwa endotoxin ndi kuyesa.Zogulitsa zathu zimaphatikizapo kugwiritsa ntchito kafukufuku wa labotalezida zochotsera endotoxins ndipo atha kukulitsidwa mpaka magawo opangira mafakitale.Lipopolysaccharide (LPS) ndi bakiteriya endotoxin komanso gawo lalikulu la ma cell a mabakiteriya a gram-negative.Recombinantprotein yochokera kuE.colius nthawi zambiri imakhala ndi ma endotoxins ambiri.Kuchotsa ma endotoxins awa ndikofunikira kwambiri pamayendedwe akumunsi.

EtEraser HP Endotoxin Removal Kitadapangidwa kuti achotse kuipitsidwa kwa endotoxin ku njira zamadzi.Makitiwa ali ndi ma endotoxin kuchotsa resinbinds kuti achepetse kuchuluka kwa endotoxin mu zitsanzo zamapuloteni ndi ≥99% pasanathe 2hours.

Chida ichi chochotsa endotoxin chitha kugwiritsidwa ntchito mu mapuloteni, DNA/RNA, polysaccharide ndi zitsanzo zina zamoyo.

2.Zomwe zimapangidwira

◆ kukhazikika kwakukulu - sikumakhudza ntchito za zitsanzo zambiri zamoyo

◆ kuchira kwa mapuloteni apamwamba -> 95% kuchira kwa mapuloteni kwa zitsanzo zamapuloteni

◆ Kuchotsa kwakukulu - chotsani> 99% endotoxin, mlingo wa endotoxin mu zitsanzo ukhoza kukhala wocheperapo 0.1 EU/mlafter endotoxin kuchotsa ndondomeko

◆ ntchito zosiyanasiyana - zitha kugwiritsidwa ntchito pochotsa endotoxin pamapuloteni, ma peptides, ma antibodies, katemera, polysaccharide ndi zitsanzo zina zamoyo.

EtEraserHP High Efficient endotoxin kuchotsa zida zimagwiritsa ntchito immobilized affinity ligandmodified PMB kumanga ndi kuchotsa endotoxin mu njira yamadzi.Ligand yosinthidwaPMB (polymyxin B) ndi ligand yomwe imamanga endotoxin yapamwamba kwambiri.Thecolumn ili ndi kuchuluka kwa mapuloteni apamwamba kwambiri kuposa 95%.Mulingo wa endotoxin pachitsanzo utha kukhala wochepera 0.1 EU/ml pambuyo pochotsa endotoxin.

Kitin ikuphatikiza ndime yochotsa endotoxin yodzaza kale ndi 1.5 ml, Buffer Equilibration, Regeneration Buffer ndi chubu chosonkhanitsira chopanda pyrogen ndi malangizo.Mzerewu uli ndi mphamvu zomangirira kwambiri> 2, 000, 000 EU / ml.Izi zitha kugwiritsidwanso ntchito mpaka kasanu ngati zitapangidwanso bwino.The affinity resin ilipo inslurry ndipo ikhoza kukwezedwa mu biopharmaceutical process.