Kugwiritsa Ntchito Chromogenic Technique pa Bacterial Endotoxins Test

Njira ya Chromogenic ndi imodzi mwa njira zitatu zomwe zilinso ndi njira ya gel-clot ndi njira ya turbidimetric kuti azindikire kapena kuwerengera ma endotoxins kuchokera ku mabakiteriya a Gram-negative pogwiritsa ntchito amoebocyte lysate yotengedwa m'magazi a buluu a nkhanu (Limulus polyphemus kapena Tachypleus tridentatus).Itha kugawidwa ngati kuyesa kwa endpoint-chromogenic kapena kinetic-chromogenic assay kutengera mfundo yomwe yagwiritsidwa ntchito.

Mfundo yake ndi yakuti: amebocyte lysate imakhala ndi ma serine protease enzymes (ma proenzymes) omwe amatha kuyambitsidwa ndi ma endotoxins a bakiteriya.Endotoxins imayendetsa ma proenzymes kuti apange ma enzymes (otchedwa coagulase), yotsirizirayi imathandizira kugawanika kwa gawo lapansi lopanda mtundu, ndikutulutsa pNA yamtundu wachikasu.PNA yotulutsidwa imatha kuyezedwa pazithunzi pa 405nm.Ndipo kuyamwa kumalumikizidwa bwino ndi kuchuluka kwa endotoxin, ndiye kuti kuchuluka kwa endotoxin kumatha kuwerengedwa moyenerera.