Chromogenic TAL Assay (Chromogenic endotoxin test assay)

Chromogenic TAL Assay (Chromogenic endotoxin test assay)

TAL reagent ndi lyophilized amebocyte lysate yomwe imatengedwa m'magazi a buluu a Limulus polyphemus kapena Tachypleus tridentatus.

Endotoxins ndi amphiphilic lipopolysaccharides (LPS) yomwe ili mu membrane yakunja ya mabakiteriya a gram-negative.Zopangira za makolo zomwe zili ndi ma pyrogens kuphatikiza LPS zimatha kuyambitsa kutentha thupi, kuyambitsa kuyankha kotupa, kugwedezeka, kulephera kwa ziwalo ndi kufa mwa munthu.Chifukwa chake, maiko padziko lonse lapansi apanga malamulo, omwe amafuna kuti mankhwala aliwonse omwe amati ndi osabala komanso osakhala a pyrogenic ayesedwe asanatulutsidwe.Kuyeza kwa gel-clot TAL kudapangidwa koyamba kuyesa ma endotoxins a bakiteriya (ie BET).Komabe, njira zina zapamwamba za kuyesa kwa TAL zatulukira.Ndipo njirazi sizidzangozindikira komanso kuwerengera kukhalapo kwa endotoxins pachitsanzo.

Kupatula njira ya gel-clot, njira za BET zilinso ndi njira ya turbidimetric ndi njira ya chromogenic.

Bioendo, wodzipereka pakuzindikira endotoxin, ndiye wopanga akatswiri kuti apange kuyesa kwa chromogenic TAL.Bioendo^TMEC Endotoxin Test Kit (End-point Chromogenic Assay) imapereka muyeso wachangu wa endotoxin quantification.Timaperekanso Bioendo^TMKC Endotoxin Test Kit (Kinetic Chromogenic Assay) ndi owerenga ma incubation microplate ELx808IULALXH, omwe angatsimikizire kudalirika komanso kuchita bwino pazoyeserera zanu.