Kugula kalozera wa Bioendo End-point Chromogenic LAL Test Assay Kit

Malangizoza Bioendo End-point Chromogenic LAL Test Assay Kits:

TAL reagent, mwachitsanzo, lyophilized amebocyte lysate yotengedwa m'magazi a buluu a nkhanu (Limulus polyphemus kapena Tachypleus tridentatus), amagwiritsidwa ntchito nthawi zonse kuyesa ma endotoxins a bakiteriya.

Ku Bioendo, timapanga zida kuti tichite mayeso omaliza a chromogenic TAL assay / LAL, omwe amaphatikiza ma reagents ofunikira pa BET kudzera pakusintha kwamitundu, ndikulola kasitomala kupeza zotsatira zolondola pakanthawi kochepa.

Mfundo zakuzindikira kwa chromogenic ndi TAL reagent ndi izi: ma enzymatic reaction zimachitika pamene ma endotoxins a bakiteriya ayambitsa chinthu C, ndipo coagulase imapangidwa moyenerera.Kenako coagulase imagawa gawo lapansi lopanda mtundu kuti litulutse pNA yamtundu wachikasu.PNA yotulutsidwa imatha kuyesedwa pazithunzi pa 405nm.Popeza kuyamwa kumalumikizidwa bwino ndi kuchuluka kwa endotoxin, kuchuluka kwa endotoxin kumatha kuwerengedwa moyenerera.

EC64405, mwachitsanzo, BioendoEC Endotoxin Test Kit (End-point Chromogenic Assay), ndi njira yomaliza ya Chromogenic TAL assay / LAL assay kit yomwe ingagwiritsidwe ntchito pozindikira kuchuluka kwa bakiteriya endotoxin yomwe ilipo mu zitsanzo.EC64405 zida zopangidwa ndi Bioendo zimalola mayeso 64, ndipo kukhudzika kumayambira 0.1 EU/ml mpaka 1 EU/ml.Bioendo EC64405 ili ndi ma reagents onse ofunikira pakuyesa komaliza kwa chromogenic.Popeza tilinso ndi zida zopanda endotoxin kapena pyrogen, tithanso kulongedza zida kutengera zomwe mukufuna.

Incubating Microplate Reader ndiyofunikira pakuwunika kwa chromogenic kumapeto.Wowerenga wathu wa microplate ELx808IULALXH (chitsanzo chatsopano chomwe chikukonzedwa ndipo chidzayambika posachedwa) chimalola zitsanzo zosiyanasiyana kuti zidziwike mu microplate ya 96-pa nthawi imodzi ndipo idzasanthula endotoxin kudziwika mosavuta komanso molondola.

End-point chromogenic LAL test assay yokhala ndi zida ziwiri, imodzi imagwiritsa ntchito owerenga ma microplate kuti awerenge zotsatira za quantify ndi pyrogen-free microplate, winayo amagwiritsa ntchito spectrophotometer wamba kuti awerenge zotsatira zake ndi machubu agalasi opanda endotoxin.

Lyophilized Amebocyte Lysate / LAL Reagent yopangidwa ndi Bioendo amapangidwa kuchokera ku amebocyte lysate opangidwa ndi magazi a nkhanu.