Chromogenic TAL Assay EC64405

TAL reagent, mwachitsanzo, lyophilized amebocyte lysate yotengedwa m'magazi a buluu a nkhanu ya horseshore (Limulus polyphemus kapena Tachypleus tridentatus), amagwiritsidwa ntchito nthawi zonse kuyesa ma endotoxins a bakiteriya.

Ku Xieman Bioendo Technology Co., Ltd., timapanga zida kuti tichite mayeso omaliza a chromogenic TAL, omwe amaphatikiza ma reagents ofunikira pa BET kudzera pakusintha kwamitundu, ndikulola kasitomala kupeza zotsatira zolondola munthawi yochepa. nthawi.

Mfundo zakuzindikira kwa chromogenic ndi TAL reagent ndi izi: enzyme zimachitika ma endotoxins a bakiteriya akayambitsa chinthu C, ndipo coagulase imapangidwa moyenerera.Kenako coagulase imagawa gawo lopanda mtundu kuti litulutse pNA yamtundu wachikasu.PNA yotulutsidwa imatha kuyesedwa pazithunzi pa 405nm.Popeza kuyamwa kumalumikizidwa bwino ndi kuchuluka kwa endotoxin, kuchuluka kwa endotoxin kumatha kuwerengedwa moyenerera.

EC64405, mwachitsanzo, Bioendo^TMEC Endotoxin Test Kit (End-point Chromogenic Assay), ndi zida zoyeserera za Chromogenic TAL zomwe zitha kugwiritsidwa ntchito kuti zizindikire kuchuluka kwa bakiteriya endotoxin yomwe ilipo mu zitsanzo.Chida cha EC64405 chopangidwa ndi Bioendo chimalola mayeso 64, ndipo kukhudzika kumayambira 0.1 EU/ml mpaka 1 EU/ml.Bioendo's EC64405 ili ndi ma reagents onse ofunikira pakuyesa komaliza kwa chromogenic.Popeza tilinso ndi zida zopanda endotoxin kapena pyrogen, titha kulongedzanso zida kutengera zomwe mukufuna.

Incubating Microplate Reader ndiyofunikira pakuwunika kwa chromogenic kumapeto.Wowerenga wathu wa microplate ELx808IULALXH amalola zitsanzo zosiyanasiyana kuti zidziwike mu microplate ya 96-pa nthawi imodzi ndipo idzasanthula kuzindikira kwa endotoxin kokha komanso molondola.