Madzi opanda endotoxin amatenga gawo lofunikira pakulondola komanso kudalirika kwa ntchito yoyesa mayeso a endotoxin.Endotoxins, yomwe imadziwikanso kuti lipopolysaccharides (LPS), ndi zinthu zapoizoni zomwe zimapezeka m'makoma a cell a mabakiteriya a gram-negative.Zowonongekazi zimatha kuvulaza anthu ndi nyama ngati sizichotsedwa kuzinthu zamankhwala monga katemera, mankhwala, ndi zida zamankhwala.
Kuti muzindikire ndikuwerengera milingo ya endotoxin molondola, kuyesa kwa endotoxin kumadalira kuyesa tcheru komwe kumafunikira kugwiritsa ntchito madzi opanda endotoxin.Madzi amtundu uwu amathandizidwa kuti achotse zizindikiro zonse za endotoxins, kuonetsetsa kuti zotsatira zabwino zomwe zimapangidwa ndi kuyesedwa zimakhala chifukwa cha kupezeka kwa endotoxin mu chitsanzo chomwe chikuyesedwa, osati chifukwa cha kuipitsidwa kwa madzi.
Kugwiritsa ntchito madzi opanda endotoxin kumathandizanso kuchepetsa zotsatira zabodza, zomwe zimatha kuchitika ngati pali kuchuluka kwa endotoxin m'madzi omwe amagwiritsidwa ntchito poyesa.Izi zitha kubweretsa zotsatira zolakwika, zomwe zitha kubweretsa kuchedwetsa kutulutsidwa kwazinthu komanso zovuta zamalamulo.
Mwachidule, madzi opanda endotoxin ndi gawo lofunikira kwambiri pakuyesa kuyesa kwa endotoxin, kuwonetsetsa kulondola komanso kudalirika kwa mayeso ovutawa.Pochepetsa chiwopsezo cha zabwino zabodza ndikuwonetsetsa kuti zotsatira zabwino zimangopangidwa pamaso pa kuipitsidwa kwenikweni kwa endotoxin, madzi opanda endotoxin amakhala ndi gawo lofunikira pakuwonetsetsa kuti mankhwala azachipatala ndi otetezeka komanso ogwira ntchito kwa odwala.
Bakiteriya endotoxin kuyesa madzi
Kusiyana pakati pa bakiteriya endotoxin kuyesa madzi ndi madzi wosabala jekeseni: pH, bakiteriya endotoxin ndi zosokoneza.
Bakiteriya endotoxin kuyesa madzi
Kusiyana pakati pa bakiteriya endotoxin kuyesa madzi ndi madzi wosabala jekeseni: pH, bakiteriya endotoxin ndi zosokoneza.
1. pH
The abwino kwambiri pH zimene anachita pakatiLAL reagentndipo endotoxin ndi 6.5-8.0.Chifukwa chake, mu mayeso a LAL, United States, Japan Pharmacopoeia ndi kope la 2015 la Chinese Pharmacopoeia likunena kuti pH yamtengo woyeserera iyenera kusinthidwa kukhala 6.0-8.0.Mtengo wa pH wa madzi pakuyesa kwa bakiteriya endotoxin nthawi zambiri umayendetsedwa pa 5.0-7.0;pH mtengo wamadzi wosabala wa jakisoni uyenera kuyendetsedwa pa 5.0-7.0.Popeza mankhwala ambiri ndi ofooka acidic, pH mtengo wamadzi woyezetsa bakiteriya endotoxin ndi yabwino pa kuyesa kwa endotoxin kapena Lyophilized amebocyte lysate test assay.
2. Bakiteriya Endotoxin
Kuchuluka kwa endotoxin m'madzi kwa kuyesa kwa bakiteriya endotoxin kuyenera kukhala osachepera 0.015EU pa 1ml, ndipo kuchuluka kwa endotoxin m'madzi kwa kuyesa kwa bakiteriya endotoxin m'njira zambiri kuyenera kukhala kosakwana 0.005EU pa 1ml;Madzi osabala a jakisoni akuyenera kukhala osakwana 0.25 EU a endotoxin pa 1ml.
Endotoxin m'madzi yoyesa bakiteriya endotoxin iyenera kukhala yotsika mokwanira kuti isakhudze zotsatira zoyesa.Ngati madzi osabala a jakisoni agwiritsidwa ntchito m'malo mwa madzi oyesera a mayeso a Endotoxin, chifukwa cha kuchuluka kwa endotoxin m'madzi osabala a jakisoni, madzi osabala a jakisoni ndi Kuchuluka kwa endotoxin pachitsanzo choyesedwa kungayambitse zabodza, kubweretsa kuwonongeka kwachuma mwachindunji. ku bizinesi.Chifukwa cha kusiyana kwa endotoxin zomwe zili mu endotoxin, sizingatheke kugwiritsa ntchito madzi osabala jekeseni m'malo mwa madzi oyendera poyesa kuyesa kwa endotoxin kapena Lyophilized amebocyte lysate test assay.
3. Zosokoneza
Madzi oyezetsa mabakiteriya endotoxin sayenera kusokoneza reagent ya LAL, kuwongolera muyezo wa endotoxin ndi mayeso a LAL;palibe kufunikira kwa madzi osabala a jakisoni.Madzi osabala a jakisoni amafunikira chitetezo ndi kukhazikika, koma kodi madzi osabala a jakisoni angakhudze ntchito ndi kukhazikika kwa endotoxin yolamulira mabakiteriya?Kodi Madzi Osabala jekeseni Amawonjezera Kapena Amaletsa Mayeso a endotoxin?Ndi anthu ochepa amene achita kafukufuku wa nthawi yaitali pa izi.Zatsimikiziridwa kudzera mu kafukufuku kuti madzi ena osabala a jakisoni amalepheretsa kwambiri kuyezetsa kwa LAL.Ngati madzi osabala a jakisoni agwiritsidwa ntchito m'malo mwa madzi oyeserera a LAL, zolakwika zabodza zitha kuchitika, zomwe zimapangitsa kuti asazindikire endotoxin, zomwe zimawopseza chitetezo chamankhwala mwachindunji.Chifukwa cha kukhalapo kwa zinthu zosokoneza za madzi osabala jekeseni, sizotheka kugwiritsa ntchito madzi osabala pobaya jekeseni m'malo moyendera madzi oyezetsa a LAL.
Ngati kulondola kwa madzi ochapira, njira yotsuka ndi madzi oyesera zitha kutsimikiziridwa, kuthekera kuti kuwongolera kwabwino mu mayeso a Limulus sikungakhazikitsidwe kwenikweni kulibe, pokhapokha ngati muyezo womwe umagwiritsidwa ntchito suli wokhazikika.Kuti tiwonetsetse kuti zotsatira za mayesowa ndi zolondola, tiyenera:
a.Zodziwika bwino ndi miyezo ndi machitidwe amakampani;
b.Gwiritsani ntchito mankhwala oyenerera ndi zinthu zoyenera;
c.Gwirani ntchito motsatira ndondomeko zoyendetsera ntchito.
Nthawi yotumiza: Jul-26-2023
