Mu ntchito yabacterial endotoxin test assay, kugwiritsa ntchito madzi opanda endotoxin ndikofunikira kuti apewe kuipitsidwa.Kukhalapo kwa endotoxins m'madzi kumatha kubweretsa zotsatira zolakwika komanso kusokoneza zotsatira za mayeso.Apa ndipamene madzi a Lyophilized Amebocyte Lysate (LAL) reagent ndi bakiteriya endotoxin test (BET) amayamba kuseweredwa.Madzi opangidwa mwapaderawa ndi ofunikira powonetsetsa kudalirika komanso kulondola kwa kuyezetsa kwa endotoxin m'mafakitale osiyanasiyana, kuphatikiza mankhwala, zida zamankhwala, malo opangira kafukufuku ndi zina zotero.
TheLAL reagent madzindi madzi oyeretsedwa kwambiri omwe amapangidwa kuti agwiritsidwe ntchito mu mayeso a LAL a endotoxins.Madziwa amapangidwa mokhazikika kuti atsimikizire kuti alibe endotoxins, zomwe zitha kusokoneza zotsatira za mayeso.Kusapezeka kwa ma endotoxins m'madzi a LAL reagent ndikofunikira pakuwonetsetsa kukhudzika komanso kutsimikizika kwa mayeso a LAL, ndikupangitsa kuti ikhale chisankho choyenera kuti azindikire endotoxin.
Momwemonso, madzi a BET ndiwonso gawo lofunikira pakuyesa kwa bakiteriya endotoxin.Madziwa amakonzedwa mwachindunji ndikuyesedwa kuti atsimikizire kuti alibe ma endotoxins ndi zowononga zina zomwe zingakhudze kulondola kwa mayeso.Kugwiritsa ntchito madzi a BET poyesa kuyesa kwa endotoxin ndikofunikira kuti mupeze zotsatira zodalirika komanso zobwereketsa, chifukwa zimachotsa chiwopsezo chabodza kapena zolakwika zabodza zomwe zingachitike chifukwa cha kupezeka kwa endotoxins m'madzi okhazikika.
Kufunika kogwiritsa ntchito madzi opanda endotoxin pakuyesa kuyesa kwa endotoxin sikungapitiritsidwe.Kulondola ndi kudalirika kwa zotsatira zoyezetsa zimadalira ubwino wa madzi ogwiritsidwa ntchito.Kukhalapo kwa endotoxins m'madzi kumatha kupangitsa kuti anthu awerenge zabodza, zomwe zitha kukhala ndi zotsatirapo zoyipa m'mafakitale momwe kuyezetsa kwa endotoxin ndikofunikira pakuwonetsetsa kuti zinthu zili zotetezeka komanso zogwira mtima.Chifukwa chake, kuyika ndalama m'madzi a LAL reagent kapena madzi a BET ndikofunikira kuti musunge kukhulupirika kwa kuyesa kwa endotoxin ndikuwonetsetsa kuti zinthu zili bwino komanso chitetezo.
Pomaliza, kugwiritsa ntchito madzi opanda endotoxin, monga madzi a LAL reagent ndi madzi a BET, ndikofunikira pakuyesa kuyesa kwa bakiteriya endotoxin.Madzi opangidwa mwapaderawa adapangidwa kuti athetse chiwopsezo choipitsidwa ndikuwonetsetsa kulondola komanso kudalirika kwa kuyesa kwa endotoxin.Pogwiritsa ntchito madziwa, mafakitale amatha kuyesa kuyesa kwa endotoxin molimba mtima popanda kuopa zotsatira zolakwika chifukwa cha kupezeka kwa endotoxins m'madzi.Pamapeto pake, kugwiritsa ntchito madzi a LAL reagent ndi madzi a BET ndikofunikira kuti akhazikitse miyezo yapamwamba kwambiri komanso chitetezo m'mafakitale omwe kuyezetsa kwa endotoxin ndikofunikira kwambiri.
Mukayesa kuyesa kwa bakiteriya endotoxin, ndikofunikira kugwiritsa ntchito madzi opanda endotoxin kuti muwonetsetse zolondola komanso zodalirika.
Endotoxins ndi zigawo zosasunthika za khoma la cell za mabakiteriya a gram-negative, ndipo zimatha kuyambitsa kutentha thupi, kunjenjemera, ngakhale kufa mwa anthu ndi nyama.
Chifukwa chake, ndikofunikira kugwiritsa ntchito madzi omwe alibe endotoxins poyesa.
Pali mitundu ingapo yamadzi yomwe ingagwiritsidwe ntchito poyesa kuyesa kwa bakiteriya endotoxin, kuphatikiza madzi a LAL reagent, madzi a TAL reagent, ndi madzi okhala ndi chithandizo cha depyrogenation.Iliyonse mwa mitundu iyi yamadzi imapangidwa kuti iwonetsetse kuti ma endotoxins palibe, motero amatsimikizira kulondola kwa zotsatira zoyesa.
Madzi a LAL reagent ndi madzi omwe adayesedwa mwachindunji ndikutsimikiziridwa kuti alibe ma endotoxins.Madziwa amagwiritsidwa ntchito kwambiri mu Lyophilized Amebocyte Lysate (LAL) assay, yomwe ndi njira yodziwika kwambiri yodziwira endotoxins.Pogwiritsa ntchito madzi a LAL reagent poyesa, ofufuza akhoza kukhala otsimikiza kuti madziwo sakuthandiza pa zotsatira zabodza kapena zabodza.
Mofananamo, madzi a TAL reagent ndi madzi omwe adayesedwa mwachindunji ndikutsimikiziridwa kuti alibe endotoxins.Madziwa amagwiritsidwa ntchito kwambiri poyesa Tachypleus Amebocyte Lysate (TAL), njira ina yodziwika bwino yodziwira endotoxins.Pogwiritsa ntchito madzi a TAL reagent poyesa, ochita kafukufuku akhoza kukhala otsimikiza kuti madziwo sakuthandizira ku zotsatira zabodza kapena zabodza.
Madzi okhala ndi chithandizo cha depyrogenation ndi njira ina yowonetsetsa kuti madzi omwe amagwiritsidwa ntchito poyesa mabakiteriya endotoxin alibe ma endotoxins.Chithandizo cha depyrogenation chimaphatikizapo kuchotsa kapena kuyambitsa ma pyrogens, kuphatikizapo endotoxins, m'madzi.Izi zitha kutheka kudzera njira monga kusefera, distillation, kapena mankhwala.Pogwiritsa ntchito madzi omwe ali ndi chithandizo cha depyrogenation mu kuyesa, ochita kafukufuku akhoza kukhala otsimikiza kuti madzi okhawo sakuthandizira ku zotsatira zabodza kapena zabodza.
Chifukwa chake, ndichifukwa chiyani kuli kofunika kugwiritsa ntchito madzi opanda endotoxin mu kuyesa kwa bakiteriya endotoxin?Kukhalapo kwa endotoxins m'madzi omwe amagwiritsidwa ntchito poyesa kungayambitse zotsatira zolakwika, zomwe zingakhale ndi zotsatira zoopsa pa kafukufuku ndi ntchito zachipatala.Mwachitsanzo, ngati ma endotoxins amapezeka m'madzi, amatha kubweretsa zotsatira zabodza, zomwe zimasonyeza kukhalapo kwa endotoxins pamene palibe kwenikweni.Izi zitha kubweretsa nkhawa yosafunikira komanso kugwiritsa ntchito molakwika chuma kuti athetse vuto lomwe kulibe.
Mosiyana ndi zimenezi, ngati endotoxins ilipo m'madzi ndipo imapita mosadziwika, ikhoza kubweretsa zotsatira zabodza, zomwe zimasonyeza kuti endotoxins palibe pamene alipo.Izi zitha kuyambitsa kutulutsa kwazinthu zoipitsidwa, kuyika thanzi la anthu ndi nyama pachiwopsezo.
Kuphatikiza pa zomwe zingakhudze kulondola kwa zotsatira zoyesa, kugwiritsa ntchito madzi omwe alibe endotoxin kungakhudzenso momwe mayesowo amachitira.Endotoxins imatha kusokoneza ma reagents ndi zida zomwe zimagwiritsidwa ntchito poyesa, zomwe zimapangitsa kuti pakhale zotsatira zosadalirika kapena zosagwirizana.Pogwiritsa ntchito madzi opanda endotoxin, ochita kafukufuku amatha kuchepetsa zoopsazi ndikuwonetsetsa kuti kuyesedwa kumachitidwa pansi pazikhalidwe zodalirika.
Pamapeto pake, kuwonetsetsa kuti madzi omwe amagwiritsidwa ntchito poyesa kuyesa kwa bakiteriya endotoxin alibe endotoxins ndikofunikira kuti asunge kukhulupirika kwa zotsatira zoyesa.Kaya akugwiritsa ntchito madzi a LAL reagent, madzi a TAL reagent, kapena madzi omwe ali ndi chithandizo cha depyrogenation, ochita kafukufuku amatha kuchitapo kanthu kuti awonetsetse kuti madziwo sakuthandizira zolakwika kapena zosagwirizana ndi zotsatira zoyesa.Pochita zimenezi, akhoza kukhala ndi chidaliro pa kutsimikizika kwa zomwe apeza ndipo akhoza kupanga zisankho zomveka potengera zotsatira za kuyesa.
Nthawi yotumiza: Jan-26-2024
