Kuyeza kwa bakiteriya endotoxin (BET) kumachitika m'malo opangira ma labotale ambiri amakono molamulidwa ngati chinthu chofunikira kupewa kusokonezedwa.
Zoyeneranjira ya asepticndizofunikira pokonzekera ndi kuchepetsa miyezo ndi kasamalidwe ka zitsanzo.Zovalakuchita kunja kwa zida za labotale zodzitetezera (PPE) Zofunikira sizodetsa nkhawa pokhapokha ngati chinthu chomwe chikuyesedwa chikufuna kuwunikira mosamala zachitetezo chifukwa cha kawopsedwe kapena matenda.Magolovesiiyenera kukhala yopanda TALC, chifukwa TALC ikhoza kukhala ndi ma endotoxins ofunikira.Mbale owerenga, osambira madzi, ndi youma kutentha midadadaZomwe zimagwiritsidwa ntchito poyatsira zitsanzo ziyenera kukhala pa benchi ya labotale kutali ndi ma ducts otentha, mpweya wabwino, ndi mpweya (HVAC), kugwedezeka kwakukulu, ndi kuchuluka kwa ma labotale komwe kungakhudze zotsatira za mayeso.Zitsanzo za nthawi ndi zikhalidweziyenera kutsimikiziridwa ndi kulembedwa pambuyo pake, ngati kuli kofunikira, kuti zitsimikizire kuti zotsatira zolondola zingathe kupangidwa mu nthawi yoyenera.
Mwachitsanzo, ngati labotale ilandira madzi a jakisoni (WFI) kapena sampuli yomwe ikugwiritsidwa ntchito, ikuyenera kukhala mufiriji kapena ingakhale pa kutentha kwa chipinda, ndipo kwa nthawi yayitali bwanji?Asanayesedwe, tikulimbikitsidwa kuti chidebe choyambirira (zi)chitsanzo chisakanizidwe mokwanira musanachotse ma aliquot (ma) mayeso kuti ayesedwe mwachindunji kapena kuchepetsedwa motsatira.
Mayeso a Bioendo Bacterial Endotoxin Test, zoyesererazo zikuphatikizanjira yothetsera gel osakanizaendotoxin test assay ndiquantitative endotoxin test assay, gel clot njira endotoxin test assay ndi khalidwe endotoxin kuzindikira, kuyesera izi kumafuna consumables ndi depyrogenation processing, monga endotoxin free reaction machubu, dilution chubu ndi pyrogen ufulu malangizo;Kuzindikira kwa endotoxin kochulukira kumakhala ndi mayeso a kinetic chromogenic endotoxin, kuyesa kwa kinetic turbidimetric endotoxin, kuyesaku kumafuna zogwiritsira ntchito ziyenera kukumana ndi mlingo wapamwamba wa endotoxins wocheperako.0.005EU/ml( 0.001EU/ml), monga machubu opanda endotoxin, malangizo aulere a pyrogen, ndi ma microplates opanda pyrogen, ngakhale pyrogen free revoir.Mwa njira, ngati zitsanzo mankhwala, chidebe kukhala endotoxin wopanda chitsanzo botolo.
Poyesa endotoxin, zosokoneza zimatha kuchokera kuzinthu zosiyanasiyana, monga zigawo za matrix, zoyeserera zoyeserera, kapena zida.
Pofuna kupewa kusokoneza kuyesera, njira zotsatirazi zikhoza kuchitidwa:
1. Kukonzekera Kwachitsanzo: Kukonzekera kwachitsanzo koyenera ndikofunikira pakuyezetsa kolondola kwa endotoxin.
Zitsanzo za matrix ziyenera kuyesedwa bwino ndikukonzedwa kuti zitsimikizire kuti zimagwirizana ndi kuyesa kwa endotoxin.
Makamaka, zinthu zosokoneza monga lipids ndi mapuloteni ziyenera kuchotsedwa kapena kuchepetsedwa pogwiritsa ntchito njira zoyenera monga kusefera kapena centrifugation.
2. Ulamuliro Wabwino ndi Woipa: Ndikofunikira kuti muphatikizepo zowongolera zabwino ndi zoyipa pakuyesa kuyang'anira kusokoneza.
Kuwongolera kwabwino kumatsimikizira magwiridwe antchito a mayeso, pomwe zowongolera zoyipa zimazindikira kuipitsidwa kulikonse kapena kusokoneza kwa zigawo zoyeserera.
3. Kuwongolera Ubwino: Kuwongolera kwaubwino kuyenera kuchitidwa pa ma reagents, zida, ndi madzi omwe amagwiritsidwa ntchito poyesa.
Izi zimatsimikizira kuti ma reagents alibe kuipitsidwa kwa endotoxin ndipo akugwira ntchito moyenera.
4. Kuyimilira: Kuyesa kuyenera kukhala kofanana kuti zitsimikizire kuti zotsatira zonse zikufanana ndi kubwereza.
Izi zimaphatikizapo kugwiritsa ntchito kapindika wokhazikika poyesa kuyesa komanso kugwiritsa ntchito njira zofananira pokonzekera, kuyika, ndi kuzindikira.
5. Kutsimikizika: Kuyesaku kuyenera kutsimikiziridwa kuti kuwonetsetsa kuti ndi yeniyeni, yotsimikizika, komanso yodalirika.
Izi zimaphatikizapo kuyesa zitsanzo zingapo, kuphatikiza zomwe zimadziwika kuti zili ndi endotoxin, kuti mudziwe zolondola komanso zolondola za mayesowo.
Potsatira izi, kusokoneza kumatha kuchepetsedwa, ndipo kuyezetsa kolondola kwa endotoxin kumatha kuchitika.
Nthawi yotumiza: Dec-01-2022
