KCET-Kinetic Chromogenic Endotoxin Test Assay (Chromogenic endotoxin test assay ndi njira yofunikira ya zitsanzo zosokoneza.)
Kuyesa kwa kinetic chromogenic endotoxin (KCT kapena KCET) ndi njira yomwe imagwiritsidwa ntchito kuzindikira kukhalapo kwa endotoxins pachitsanzo.
Endotoxins ndi zinthu zapoizoni zomwe zimapezeka m'makoma a cell a mitundu ina ya mabakiteriya, kuphatikiza mabakiteriya opanda gram monga Escherichia coli ndi Salmonella.Mu kuyesa kwa KCET, gawo laling'ono la chromogenic limawonjezedwa ku zitsanzo, zomwe zimakhudzidwa ndi endotoxins iliyonse yomwe ilipo kuti ipange kusintha kwa mtundu.
Mlingo wa kukula kwa mtundu umayang'aniridwa pakapita nthawi pogwiritsa ntchito spectrophotometer, ndipo kuchuluka kwa endotoxin pachitsanzo kumawerengedwa kutengera mulingo uwu.
Kuyesa kwa KCT ndi njira yotchuka yodziwira ma endotoxins muzamankhwala, zida zamankhwala, ndi zinthu zina zomwe zimakumana ndi thupi la munthu.Ndi mayeso ozindikira komanso odalirika omwe amatha kudziwa ngakhale ochepa kwambiri a endotoxin, ndikupangitsa kuti ikhale chida chofunikira chowonetsetsa kuti zinthuzi zili zotetezeka.
TAL/LAL reagent ndi lyophilized amebocyte lysate yomwe imatengedwa m'magazi a buluu a Limulus polyphemus kapena Tachypleus tridentatus.
Endotoxins ndi amphiphilic lipopolysaccharides (LPS) yomwe ili mu membrane yakunja ya mabakiteriya a gram-negative.Zopangira za makolo zomwe zili ndi ma pyrogens kuphatikiza LPS zimatha kuyambitsa kutentha thupi, kuyambitsa kuyankha kotupa, kugwedezeka, kulephera kwa ziwalo ndi kufa mwa munthu.
Chifukwa chake, maiko padziko lonse lapansi apanga malamulo, omwe amafuna kuti mankhwala aliwonse omwe amati ndi osabala komanso osakhala a pyrogenic ayesedwe asanatulutsidwe.Kuyeza kwa gel-clot TAL kudapangidwa koyamba kuyesa ma endotoxins a bakiteriya (ie BET).
Komabe, njira zina zapamwamba za kuyesa kwa TAL zatulukira.Ndipo njirazi sizidzangozindikira komanso kuwerengera kukhalapo kwa endotoxins pachitsanzo.Kupatula njira ya gel-clot, njira za BET zilinso ndi njira ya turbidimetric ndi njira ya chromogenic.Bioendo, yodzipereka pakuzindikira endotoxin, ndiye wopanga akatswiri kuti apange kuyesa kwa chromogenic TAL/LAL.
Bioendo EC Endotoxin Test Kit (End-point Chromogenic Assay) imapereka muyeso wachangu wa endotoxin quantification.
Timaperekanso Bioendo KC Endotoxin Test Kit (Kinetic Chromogenic Assay) komanso owerenga ma incubation microplate ELx808IU-SN, omwe angatsimikizire kudalirika komanso kuchita bwino pazoyeserera zanu.
Kodi mawonekedwe akinetic chromogenic endotoxin test assaykuyesa endotoxins mu zitsanzo?
Kinetic chromogenic endotoxin test assay ndi njira ina yomwe imagwiritsidwa ntchito poyesa ma endotoxins mu zitsanzo.Ili ndi zinthu zingapo:
1. Kuyeza kwa Kinetic: Mofanana ndi turbidimetric assay, kinetic chromogenic assay imaphatikizapo kuyeza kwa kinetic.Zimadalira zomwe zimachitika pakati pa endotoxins ndi gawo lapansi la chromogenic kuti apange chinthu chamitundu.Kusintha kwa kukula kwa mtundu pakapita nthawi kumawunikidwa, kulola kuchulukitsidwa kwa kuchuluka kwa endotoxin mu zitsanzo.
2. Kumverera kwakukulu: The kinetic chromogenic assay ndi yovuta kwambiri ndipo imatha kuzindikira ma endotoxins otsika mu zitsanzo.Imatha kuyeza molondola kuchuluka kwa endotoxin, ngakhale pamilingo yotsika kwambiri, kuonetsetsa kuti ikupezeka modalirika komanso kuchuluka kwake.
3. Kusiyanasiyana kosiyanasiyana: Kuyesa kumakhala ndi mitundu yosiyanasiyana, kulola kuyeza kuchuluka kwa endotoxin kudera lalikulu.Izi zikutanthauza kuti imatha kuyesa zitsanzo zokhala ndi ma endotoxins osiyanasiyana, kutengera zotsika komanso zochulukira popanda kufunikira kwa kuchepetsedwa kwa zitsanzo kapena kukhazikika.
4. Zotsatira zofulumira: The kinetic chromogenic assay imapereka zotsatira zofulumira poyerekeza ndi njira zachikhalidwe.Nthawi zambiri imakhala ndi nthawi yayifupi yoyesa, yomwe imathandizira kuyesa mwachangu komanso kusanthula zitsanzo.Kukula kwamtundu kumatha kuyang'aniridwa munthawi yeniyeni, ndipo zotsatira zake zimatha kupezeka mkati mwa mphindi zingapo mpaka maola angapo, kutengera zida zoyeserera ndi zida zomwe zimagwiritsidwa ntchito.
5. Zodzipangira zokha ndi zoyimira: Kuyesa kutha kuchitidwa pogwiritsa ntchito makina odzipangira okha, monga owerenga ma microplate kapena
endotoxin-enieni analyzer.Izi zimalola kuyesa kwapamwamba ndikuwonetsetsa kuti miyeso yokhazikika komanso yokhazikika, kuchepetsa zolakwika za anthu ndikuwonjezera mphamvu.
6. Kugwirizana ndi mitundu yosiyanasiyana ya zitsanzo: The kinetic chromogenic assay imagwirizana ndi mitundu yambiri ya zitsanzo, kuphatikizapo mankhwala, zipangizo zamankhwala, biologics, ndi zitsanzo za madzi.Ndi njira yosunthika yomwe ingagwiritsidwe ntchito m'mafakitale osiyanasiyana ndikugwiritsa ntchito komwe kuyezetsa kwa endotoxin kumafunika.
Ponseponse, kuyesa kwa kinetic chromogenic endotoxin kumapereka njira yomvera, yachangu, komanso yodalirika yodziwira ndikuwerengera
endotoxins mu zitsanzo.Amagwiritsidwa ntchito kwambiri m'mafakitale azamankhwala, biotechnology, ndi chisamaliro chaumoyo pakuwongolera ndi chitetezo.
Zolinga zowunika.
Nthawi yotumiza: Jul-29-2019
