Endotoxin Test Assay ndi Lyophilized Amebocyte Lysate (LAL Reagent)

Endotoxin Test Assay ndi Lyophilized Amebocyte Lysate (LAL Reagent)

Zithunzi za LAL: Lyophilized amebocyte lysate (LAL) ndi madzi amadzimadzi a m'magazi (amebocytes) kuchokera ku nkhanu ya Atlantic horseshoe.
TAL Reagents: TAL reagent ndi chotulutsa chamadzi chamagazi kuchokera ku Tachypleus tridentatus.
Pakalipano, kupanga kwakukulu kwa ma reagents a LAL/TAL ali ku United States ndi China.

Gel clot endotoxin test assay, njira iyi ikadali yogwiritsidwa ntchito kwambiri padziko lonse lapansi, kuti akwaniritse zofunikira zachitetezo chamankhwala a jakisoni a Anthu.
PANOPA Bioendo imapanga ndikupereka zopangira za gel clot LAL kuphatikiza ma ampule agalasi oyeserera ndi mbale zoyesera zambiri.
https://www.bioendo.com/gel-clot-endotoxin-assay/ G01, GS44, G02, G17 ndi G52
Ili ndiye njira yachuma yodziwira ma endotoxins abwino pakuyezetsa mankhwala.Makamaka mankhwala jekeseni kapena parenteral mankhwala kuyesa endotoxins mu WFI, API kapena anamaliza mankhwala mankhwala.Endotoxin test assay ndiyofunika kwambiri pochitapo kanthu, imafunika wogwiritsa ntchito waluso kuti athane ndi kuyezetsa magazi kwa gel kuti atsimikizire zolondola.

Yankho lathunthu laG52Kuyesa kwa Endotoxin:
Zotsatira LAL
Control Standard Endotoxin
BET Madzi
Malangizo a pipette wopanda endotoxin
Machubu agalasi opanda endotoxin, kuphatikizapo dilution operation ndi reaction chubu.
Makulitsidwe chida, amalangiza osamba madzi kapena youma chofungatira kutentha.Zipangizo zonse zoyatsira zimafuna kutentha molondola.
Zonse zogwiritsidwa ntchito kukhudza kuyesa kwa LAL komwe kumakwaniritsa mulingo wopanda endotoxin ”<0.005EU/ml ” .
Malo oyesera ayenera kukhala ogwirizana ndi kuzindikirika kwa endotoxin.

Samalani ku:
Zogula zamapulasitiki monga malangizo a pipette kapena mbale za multiwell zimagwiritsidwa ntchito kwambiri poyesa endotoxin.The harmonized Pharmacopoeias (USP/CP) amafuna kuti zinthu zilizonse za pulasitiki zogwiritsidwa ntchito ndi machubu agalasi ziyenera kukhala zopanda endotoxin zodziwika komanso zofunikira koma nthawi zambiri osaganiziridwa, ziyenera kukhala mulingo wopanda zosokoneza.

Momwe mungagwiritsire ntchito kuyesa kwa endotoxin kuti muwone ma endotoxins mu zitsanzo?
Choyamba, kuyesa kutsimikizira kukhudzidwa kwa lysate kuyenera kuchitidwa.Kutsimikizira kukhudzika ndi chimodzimodzi kwa chizindikiro zizindikiro.
Pakuwunika kwachitsanzo, chitani mayeso a pre-interference endotoxin test.
Kuyesa kuyesa kwa endotoxin kwa "kusokoneza mayeso".
Kuti mugwiritse ntchito mayeso oletsa kuti muwone momwe ma endotoxins alili pachitsanzocho.

Mukayesa kuyesa kutsimikizira za kukhudzidwa kwa lysate, zotsatira zake zimakhala zachilendo, 2 Lamada havenot gel mapangidwe?
Yang'anani ngati njira yokonzekera yowongolera mabakiteriya endotoxin ndiyolondola.
Kusakaniza kwa Vortex kumafunika pa diluent iliyonse (onani buku la malangizo kapena kutchedwa kuyika kwa endotoxin yowongolera mwatsatanetsatane).
Kuti muwone ngati lysate reagent ikufanana ndi endotoxin yolamulira.

Ndibwino kuti mugwiritse ntchito madzi osamba kapena chowumitsira kutentha kwa kutentha kosalekeza kwa lysate reagent (mwachitsanzo, chofungatira kapena bokosi louma silikugwira ntchito).
Pa makulitsidwe, madzi chilengedwe mu osamba madzi kuti ali static ndi okhazikika, kugwedezeka onse amapewa.
Samalani kwambiri kusamba kwa madzi kuti muzimitse mpope wa madzi oyenda.

Poyang'ana zotsatira zake, mokoma chotsani chubu choyesera kapena magalasi ampoule kuchokera ku thermostat, pang'onopang'ono mutembenuzire madigiri 180,
mapangidwe a gel mu chubu sichidzawonongeka, ndipo sichidzachoka, zotsatira zake zinalembedwa ndi chizindikiro "+";
palibe mapangidwe a gel opangidwa kapena gel osakaniza sangathe kusungidwa ngakhale atapangidwa.
Kuzembera khoma ndi kolakwika, zotsatira zake zidalembedwa ndi chizindikiro "-".
Rapid gel clot endotoxin test Kit ndi ya gel clot endotoxin test njira.
Pakuyezetsa mwachangu kwa gel osakaniza endotoxin, kuwongolera kwabwino sikutulutsa gel?
Choyamba, gwiritsani ntchito madzi oyesa mabakiteriya endotoxin kuti mutsimikizire ma reagents a lysate, onetsetsani kuti ogwira ntchito ndi chilengedwe akwaniritsa malamulowo;

Ngati chidacho chili choyenera, ndiye kuti kuwongolera kwachitsanzo sikumapangidwira mapangidwe a gel chifukwa cha kulepheretsa kwa chitsanzocho, ndipo chitsanzocho chiyenera kukonzedwa ndikuchiritsidwa.
Njira yodziwika kwambiri yopangira zitsanzo ndi dilution.