LAL Reagent kapena TAL Reagent ya kuyesa kwa endotoxin

Limulus amebocyte lysate (LAL) kapena Tachypleus tridentatus lysate (TAL) ndi madzi otuluka m'maselo a magazi kuchokera ku nkhanu ya horseshoe.

Ndipo ma endotoxins ndi mamolekyu a hydrophobic omwe ali gawo la lipopolysaccharide complex lomwe limapanga zambiri zakunja kwa mabakiteriya a Gram-negative.Zopangidwa ndi makolo zomwe zili ndi ma pyrogens zimatha kubweretsa zotsatira zoyipa monga kutentha thupi, kunjenjemera, kulephera kwa chiwalo, ngakhale imfa.

LAL/TAL reagent imatha kuchitapo kanthu ndi bakiteriya endotoxin ndi lipopolysaccharide (LPS).Kuthekera kwa endotoxin kwa LAL kumangiriza ndikutsekeka ndizomwe zimapangitsa kuti ikhale yofunikira kwambiri kumakampani athu azamankhwala.Ichi ndichifukwa chake reagent ya LAL/TAL itha kugwiritsidwa ntchito kuti azindikire kapena kuwerengera kuchuluka kwa bakiteriya endotoxin.

Asanapezeke kuti LAL/TAL itha kugwiritsidwa ntchito poyesa ma endotoxins a bakiteriya, akalulu amagwiritsidwa ntchito kuti azindikire ndikuwerengera kuchuluka kwa endotoxins muzinthu zamankhwala.Poyerekeza ndi RPT, BET yokhala ndi LAL/TAL reagent ndiyofulumira komanso yothandiza, ndipo ndi njira yotchuka yowunikira ndende ya endotoxin m'makampani opanga mankhwala, ndi zina zotero.

Kuyesa kwa gel clot endotoxin test assay, yomwe imadziwikanso kuti Limulus Amebocyte Lysate (LAL) test, kapena yotchedwa Lyophilized Amebocyte Lysate (LAL) ndi njira yomwe imagwiritsidwa ntchito kwambiri pozindikira ndi kuwerengera ma endotoxins muzinthu zosiyanasiyana, makamaka m'makampani opanga mankhwala ndi zida zamankhwala.Imawonedwa ngati yankho lofunikira pankhani yodziwikiratu endotoxin chifukwa cha mphamvu yake komanso kuvomereza kwake.

Kuyezetsa kwa LAL kumachokera pa mfundo yakuti maselo a magazi a nkhanu za akavalo (Limulus polyphemus kapena Tachypleus tridentatus) ali ndi chinthu chotseka chomwe chimagwirizana ndi ma endotoxins a bakiteriya, zomwe zimapangitsa kuti pakhale jekeseni ngati gel.Izi zimakhudzidwa kwambiri ndi ma endotoxins, omwe ndi zigawo zapoizoni zakunja kwa mabakiteriya a gram-negative.

Pali zifukwa zingapo zomwe gel clot endotoxin test assay imatengedwa ngati yankho lofunikira pakuzindikira endotoxin:

1. Kuvomerezeka kwa Malamulo: Kuyesa kwa LAL kumazindikiridwa ndikuvomerezedwa ndi maulamuliro olamulira monga United States Pharmacopeia (USP) ndi European Pharmacopoeia (EP) monga njira yodziwika yoyesera endotoxin.Kutsatira malamulowa ndikofunikira pakuwonetsetsa chitetezo ndi mtundu wa mankhwala.

2. Kumverera ndi Kufotokozera: Kuyesa kwa LAL kumakhala ndi chidziwitso chapamwamba, chomwe chimalola kuti azindikire ma endotoxins otsika kwambiri.Imatha kuzindikira kuchuluka kwa endotoxin komwe kumatsika mpaka 0.01 endotoxin mayunitsi pa mililita (EU/mL).Kutsimikizika kwa mayeso kumatsimikizira kuti imazindikira ma endotoxins ndikuchepetsa zotsatira zabodza.

3. Mtengo Wogwira Ntchito: Kuyesa kwa gel clot endotoxin test assay nthawi zambiri kumatengedwa ngati njira yothetsera chuma poyerekeza ndi njira zina monga chromogenic kapena turbidimetric assays.Pamafunika reagents ochepa ndi zipangizo, kuchepetsa lonse kuyezetsa ndalama.Kuphatikiza apo, kupezeka kwa ma reagents okhazikika a LAL pamsika kumapangitsa kukhala kosavuta kuti ma labotale azichita mayeso.

4. Mulingo wa Makampani: Kuyesa kwa LAL kwavomerezedwa kwambiri m'makampani opanga mankhwala ndi zida zamankhwala monga njira yodziwika bwino yodziwira endotoxin.Ndi gawo lofunikira kwambiri pakuwongolera magwiridwe antchito popanga zinthu zamankhwala ndi zida zamankhwala, kuwonetsetsa kuti zikutsatira malamulo.

Komabe, ndikofunikira kudziwa kuti kuyesa kwa gel clot endotoxin kungakhale ndi malire, monga kusokonezedwa ndi zinthu zina komanso kuthekera kwa zotsatira zabodza kapena zabodza.Nthawi zina, njira zina monga ma chromogenic kapena ma turbidimetric assays atha kugwiritsidwa ntchito kuti agwirizane kapena kutsimikizira zotsatira zopezeka pamayeso a LAL.