Madzi a BET amatenga gawo lofunikira pakuyesa kwa endotoxin

Madzi Opanda Endotoxin: Kuchita Ntchito Yofunika Pakuyesa kwa Endotoxin

Chiyambi:

Kuyesa kwa Endotoxin ndi gawo lofunikira kwambiri m'mafakitale osiyanasiyana, kuphatikiza mankhwala, zida zamankhwala, ndi biotechnology.Kuzindikira kolondola komanso kodalirika kwa ma endotoxins ndikofunikira kuti zitsimikizire chitetezo chazinthu ndikutsata miyezo yoyendetsera.Chofunikira chimodzi chofunikira pakuyesa endotoxin ndikugwiritsa ntchito madzi opanda endotoxin.M'nkhaniyi, tiwona kufunika kwa madzi opanda endotoxin, ntchito yake poyesa Lyophilized Amebocyte Lysate (LAL) endotoxin test, komanso kufunika kogwiritsa ntchito madzi opanda endotoxin mu Bacterial Endotoxin Test (BET).

Kumvetsetsa Endotoxins:

Endotoxins ndi lipopolysaccharides (LPS) yomwe imapezeka kunja kwa mabakiteriya a Gram-negative.Ndiwoyimira pakati pa kutupa ndipo amatha kubweretsa zotsatira zoyipa ngati alipo muzamankhwala kapena zida zamankhwala.Chifukwa cha kuthekera kwawo koyambitsa machitidwe a pyrogenic, kuzindikira molondola komanso kuchuluka kwa endotoxins ndikofunikira.

Kuyesa kwa LAL Endotoxin:

Njira yodziwika kwambiri yoyezetsa endotoxin ndi kuyesa kwa LAL, komwe kumagwiritsa ntchito magazi a nkhanu za akavalo.Limulus polyphemus ndi Tachypleus tridentatus.Lyophilized Amebocyte Lysate (LAL) reagent imachotsedwa m'maselo amagazi a nkhanu, omwe amakhala ndi mapuloteni otsekera omwe amalowetsedwa pamaso pa endotoxins.

Udindo waMadzi Opanda Endotoxinmu Mayeso a LAL:

Madzi ndi chigawo chachikulu mu kukonzekera reagent ndi dilution masitepe LAL kuyezetsa.Komabe, ngakhale kutsata kuchuluka kwa ma endotoxins omwe amapezeka m'madzi apampopi pafupipafupi amatha kusokoneza kulondola komanso kumva kwa mayesowo.Pofuna kuthana ndi vutoli, madzi opanda endotoxin ayenera kugwiritsidwa ntchito panthawi yonse yoyezetsa.

Madzi opanda endotoxin amatenga gawo lofunikira pakuwonetsetsa kuti ma reagents omwe amagwiritsidwa ntchito mu LAL assay sayipitsidwa ndi endotoxins.Kuphatikiza apo, imalepheretsa zotsatira zabodza kapena zabodza, potero zimapatsa endotoxin yodalirika komanso yolondola.

Kusankha Madzi Oyenera Kuyesedwa kwa LAL:

Kuti mupeze madzi opanda endotoxin, njira zingapo zoyeretsera zitha kugwiritsidwa ntchito.Deionization, distillation, ndi reverse osmosis ndi njira zomwe zimagwiritsidwa ntchito pochepetsa kupezeka kwa endotoxins m'madzi.Njirazi zimachotsa zonyansa zosiyanasiyana, kuphatikiza ma endotoxins ochokera ku mabakiteriya.

Kuphatikiza apo, ndikofunikira kuwonetsetsa kuti zotengera zomwe zimagwiritsidwa ntchito posungira, kutolera, ndi kugawa madzi opanda endotoxin ndizovomerezeka komanso zopanda kuipitsidwa kwa endotoxin.Izi zikuphatikiza kugwiritsa ntchito machubu opanda endotoxin, mabotolo, ndi zosefera panthawiyi.

Kufunika kwa BET Water:

MuKuyesa kwa Bacterial Endotoxin (BET), madzi opanda endotoxin, omwe amadziwikanso kuti madzi a BET, amagwiritsidwa ntchito ngati chiwongolero choipa kuti atsimikizire kukhudzidwa ndi kutsimikizika kwa kuyesa kwa LAL.Madzi a BET ayenera kukhala ndi mlingo wosadziwika wa endotoxins, kuonetsetsa kuti ntchito iliyonse yoyezera endotoxin imachokera ku zitsanzo zoyesedwa.

Kugwiritsa ntchito madzi a BET mu mayeso a endotoxin kumagwira ntchito ngati chiwongolero chofunikira kutsimikizira mphamvu ya ma reagents a LAL, dongosolo loyesera, ndi zida.Gawo lotsimikizirali ndilofunika kuti muwone bwino kupezeka ndi kuchuluka kwa ma endotoxins mu zitsanzo zoyesedwa.

Pomaliza:

Madzi opanda endotoxin amatenga gawo lofunikira pakuzindikira kolondola komanso kodalirika kwa ma endotoxins m'mafakitale osiyanasiyana.Pakuyesa kwa LAL endotoxin, imawonetsetsa kuti ma reagents omwe amagwiritsidwa ntchito sanaipitsidwe, kupereka kuchulukira kwake.Mu BET, madzi opanda endotoxin amagwira ntchito ngati chiwongolero, kutsimikizira kukhudzika kwa kuyesa kwa LAL.Potsatira njira zoyeretsera mwamphamvu ndikugwiritsa ntchito zotengera zovomerezeka, kuthekera kwa zotsatira zabodza ndi zolakwika zitha kuchepetsedwa kwambiri.

Pamene kufunikira kwa kuyezetsa endotoxin kukukulirakulira, gawo lamadzi opanda endotoxin limakhala lofunikira kwambiri.Kugwiritsa ntchito njira zodalirika zoyeretsera madzi ndikuphatikiza njira zabwino kwambiri pakuyezetsa kudzatsimikizira chitetezo ndi kutsatiridwa kwa mankhwala, zida zamankhwala, ndi zinthu zina zomwe zimakhudzidwa ndi endotoxin.