Recombinant Factor C (rFC) kuyesandi njira yomwe imagwiritsidwa ntchito kwambiri pozindikira ma endotoxins a bakiteriya, omwe amadziwikanso kuti lipopolysaccharides (LPS), Endotoxins ndi gawo lakunja kwa mabakiteriya a Gram-negative omwe angayambitse kuyankha kwakukulu kotupa kwa nyama, kuphatikiza anthu.Kuyeza kwa rFC kumatengera kugwiritsa ntchito mawonekedwe opangidwa ndi chibadwa a Factor C, puloteni yomwe imapezeka mwachilengedwe m'magazi a nkhanu za akavalo ndipo imakhudzidwa ndi njira yotseka.Mu kuyesa kwa rFC, Factor C yophatikizanso imagwiritsidwa ntchito kuzindikira kukhalapo kwa endotoxins poyeza Poyesa zomwe zili m'magawo ong'ambika pamaso pa endotoxin.Poyerekeza ndi njira zachikhalidwe zodziwira endotoxin, monga kuyesa kwa Limulus Amebocyte Lysate (LAL) komwe kumagwiritsa ntchito magazi a nkhanu za akavalo, kuyesa kwa rFC kumaonedwa kuti ndi kovomerezeka komanso kupangidwanso, chifukwa sikudalira kugwiritsa ntchito mankhwala opangidwa ndi nyama.The rFC assay ndiyonso yokonda zachilengedwe komanso yokhazikika, chifukwa imachepetsa kufunika kosonkhanitsa ndi kugwiritsa ntchito nkhanu za akavalo pozindikira endotoxin.
Kuyesa kwa rFC kumavomerezedwa ndi olamulira, monga United States Pharmacopeia (USP), European Pharmacopoeia (EP) ndi Chinese Pharmacopoeia (CP) kuti agwiritsidwe ntchito poyesa kuwongolera kwamankhwala ndi zida zamankhwala.
Ubwino wa recombinant factor c assay
Kuyesa kwa Recombinant Factor C (rFC) kumapereka maubwino angapo kuposa njira zachikhalidwe zodziwira endotoxins, monga kuyesa kwa Limulus Amebocyte Lysate (LAL).Zina mwazabwino za rFC assay ndi:
1. Kukhazikika: Kuyesa kwa rFC ndiukadaulo wophatikizanso wa DNA womwe umagwiritsa ntchito puloteni imodzi, yomwe imatanthauzidwa ngati reagent yozindikira.Izi zimapangitsa kuti chiyesocho chikhale chokhazikika komanso chosasinthika poyerekeza ndi kuyesa kwa LAL, komwe kumadalira kugwiritsa ntchito mapuloteni osakanikirana omwe amachotsedwa m'magazi a nkhanu.
2. Kuberekanso: Kuyesa kwa rFC kumakhala ndi mphamvu zowonjezera, chifukwa zimagwiritsa ntchito puloteni imodzi, yodziwika ngati reagent yodziwira.Izi zimapangitsa kuti pakhale zotsatira zofananira, ngakhale pamagulu osiyanasiyana komanso ma reagents ambiri.
3. Kuchepetsa kugwiritsidwa ntchito kwa nyama: Kuyesa kwa rFC ndi njira yosamalira zachilengedwe komanso yokhazikika yodziwira ma endotoxins, chifukwa simafunikira kugwiritsa ntchito nyama zamoyo kapena zoperekedwa nsembe, monga nkhanu za akavalo.
4. Kutsika mtengo: Kuyesa kwa rFC nthawi zambiri kumakhala kotsika mtengo kuposa kuyesa kwa LAL, chifukwa cha kuchepa kwa kufunikira kwa nyama zamoyo komanso kukhazikika kwa kuyesako.
5. Kukhazikika: Kuyesa kwa rFC ndi kolimba ndipo kungagwiritsidwe ntchito pazinthu zosiyanasiyana, kuphatikizapo kuyesa kulamulira kwabwino kwa mankhwala, zipangizo zamankhwala, ndi zinthu zina zomwe zingakhale ndi endotoxins.
6. Chivomerezo cha malamulo: Kuyesa kwa rFC kwavomerezedwa ndi akuluakulu olamulira monga United States Pharmacopeia (USP) , European Pharmacopoeia (EP) ndi Chinese Pharmacopoeia (CP) kuti agwiritsidwe ntchito poyesa kuwongolera khalidwe la mankhwala ndi zipangizo zachipatala.Izi zimapereka chidaliro chambiri pakudalirika komanso kulondola kwa mayesowo.
Kuti akwaniritse zofuna zosiyanasiyana, Bioendo imapanganso ndikupereka njira zachikhalidwe zoyesera zida zoyeserera za gel clot endotoxin, zida zoyeserera za gel osakaniza, zida zoyeserera za endotoxin kuphatikiza "kinetic turbidimetric endotoxin test assay kitndikinetic chromogenic endotoxin test assay kit” .
Nthawi yotumiza: Feb-19-2023
