Kodi Endotoxin Ndi Chiyani

Endotoxins ndi mamolekyu ang'onoang'ono opangidwa ndi bakiteriya a hydrophobic lipopolysaccharides (LPS) omwe amapezeka mu membrane yakunja ya mabakiteriya a gram-negative.Endotoxins imakhala ndi unyolo wapakati wa polysaccharide, unyolo wa O-specific polysaccharide (O-antigen) ndi lipid compenent, Lipid A, yomwe imayambitsa poizoni.Mabakiteriya amakhetsa endotoxin wambiri pakufa kwa maselo komanso akamakula ndikugawanika.Escherichia coli imodzi ili ndi mamolekyu pafupifupi 2 miliyoni a LPS pa selo.

Endotoxin imatha kuyipitsa ma labware mosavuta, ndipo kupezeka kwake kumatha kupereka zoyeserera mu vitro komanso mu vivo.Ndipo pazinthu za parenteral, mankhwala a parenteral omwe ali ndi endotoxins kuphatikizapo LPS angayambitse kukula kwa malungo, kulowetsedwa kwa kutupa, kugwedezeka, kulephera kwa ziwalo ndi imfa mwa munthu.Pazinthu za dialysis, LPS imatha kusamutsidwa kudzera mu membrane yokhala ndi pore yayikulu ndikusefera kumbuyo kuchokera kumadzimadzi a dialysis kupita m'magazi, zovuta zotupa zimatha kuyambitsa moyenerera.

Endotoxin imadziwika ndi Lyophilized Amebocyte Lysate (TAL).Bioendo yadzipereka kuti ifufuze, kupanga ndi kupanga reagent ya TAL kwa zaka zoposa makumi anayi.Zogulitsa zathu zimaphatikiza njira zonse zomwe zimagwiritsidwa ntchito pozindikira endotoxin, zomwe ndi njira ya gel-clot, njira ya turbidimetric, ndi njira ya chromogenic.