Quantitative Endotoxin Assay Solution Ya WFI kapena Dialysate
Dialysis (Njira ya Micro Kinetic Chromogenic)
M'zaka za m'ma 1970, vuto la hemodialysis la "pyrogenic reaction" linayambika ku United States.Kafukufuku wamtsogolo adapeza kuti dialysate idayipitsidwa ndi mabakiteriya a Gram-negative.Mmene pyrogen imachitidwira ndi hemodialysis makamaka kuti pyrogen mu dialyzer ndi madzi a dialysis amaposa muyezo, ndipo pyrogen yomwe ili m'magazi a wodwalayo imawonjezeka panthawi ya dialysis, zomwe zimayambitsa zizindikiro zambiri.Pofuna kupewa zovuta za kuperewera kwa madzi kwa odwala, makonzedwe a dialysate, kutsekemera kwa dialysate, ndi madzi ogwiritsira ntchito dialyzer ndi mapaipi a magazi zonse zimafunika kugwiritsa ntchito madzi osabala, omwe ndi madzi a dialysis.Ukhondo wamadzi a dialysis umagwirizana ndi thanzi komanso moyo wa odwala ambiri omwe ali ndi vuto laimpso.Chifukwa chake, ndikofunikira kuwongolera mtundu wamadzi okhudzana ndi dialysis.
Lysate reagent yaying'ono KC yogwiritsa ntchito kinetic chromogenic yaying'ono-ukadaulo imafuna 25 μl ya zitsanzo ndi 25 μl ya Lysate reagent pamayeso aliwonse.Ili ndi mphamvu zotsutsana ndi kusokoneza komanso kukhudzidwa kwa 0.005EU/ml.Ndizoyenera kwambiri kuzindikira kwa endotoxin pazinthu zokhudzana ndi dialysis.Ili ndi kachipangizo kakang'ono kamene kamazindikira 96-well plate/slat ndi lysate test microorganism ELx808, yomwe imazindikira kuzindikira kwapang'onopang'ono kwa kinetic chromogenic endotoxin, ndipo makinawo amangozindikira ndikusanthula gawo limodzi.
Zogulitsa zofananira pakuyesa kuyesa kwa micro kinetic chromogenic endotoxin:
MKC zida: MKC0505VS;MKC0505V;MKC0505AS;MKC0505A, MPMC96.
Botolo la zitsanzo zopanda endotoxin, Catalog nambala PA10, 10ml voliyumu, yankho lalikulu la voliyumu lidzaperekedwa.
Machubu oyesera opanda endotoxin, Catalog nambala T107540 ndi T1310018
Ma microplates opanda Endotoxin (ochotsedwa / osachotsedwa), Catalog nambala MPMC96, mizere 8.
Malangizo opanda Endotoxin (1000ul ndi 250ul), Catalog nambala PT25096 kapena PT100096
Control Standard Endotoxin, Catalog nambala CSE10V
BET madzi, Catalog nambala TRW50 kapena TRW100
Wowerenga Microplate: ELx808
Timapereka zida zoyeserera za Bioendo KT endotoxin, ELx808, vortex chosakanizira ndi mizere ya microplate yopanda pyrogen kuti mupeze yankho la kuchuluka kwa endotoxin assay ya WFI kapena Dialysate.
