Machubu agalasi okhala ndi chithandizo cha depyrogenation kuti muwonetsetse kuti ndi machubu agalasi opanda endotoxin

Machubu agalasi okhala ndi depyrogenation processing ndi ofunikira pakuyesa kwa endotoxin kuti muwonetsetse kulondola komanso kudalirika kwa zotsatira zoyesa.Endotoxins ndi zigawo zokhazikika za maselo akunja kwa mabakiteriya omwe alibe gram, ndipo amatha kudwala kwambiri komanso kufa mwa anthu ngati alipo muzinthu zamankhwala kapena zida.

Kuti azindikire ma endotoxins, assay amagwiritsa ntchito ma reagents omwe ali ndi Limulus Amebocyte Lysate (LAL) kapena otchedwa Lyophilized amebocyte lysate, omwe amachokera m'maselo a magazi a nkhanu omwe amakhala ndi njira yotsekera yomwe imayendetsedwa ndi endotoxins.Komabe, machubu agalasi omwe sanachotsedwe amatha kusokoneza mayeso a LAL poyambitsa makina ake otsekera ndikupanga zotsatira zabodza.Chifukwa chake, machubu agalasi omwe amagwiritsidwa ntchito poyesa mayeso a endotoxin ayenera kuchotsedwa kuti achotse ma endotoxins aliwonse omwe angakhalepo ndikuletsa kuyambitsa kwa LAL reagent.Izi zimawonetsetsa kuti zotsatira za mayeso a endotoxin ndizolondola komanso zodalirika komanso kuti odwala samakumana ndi ma endotoxins owopsa.ndikuwonetsetsa chitetezo cha mankhwala a parenteral muzamankhwala, ma protain, cell culture, DNA ndi zina zotero.

Kufunika kwa machubu agalasi opanda endotoxin omwe amagwiritsidwa ntchito poyesa kuyesa kwa endotoxin:

Machubu agalasi opanda endotoxinNdi gawo lofunikira pakuyesa kulikonse kwa endotoxin.Machubu agalasi awa adapangidwa kuti achepetse chiwopsezo cha kuipitsidwa kwa endotoxin panthawi yoyeserera, kuwonetsetsa kuti zolondola ndi zodalirika.

Chimodzi mwazinthu zazikulu zamachubu agalasi opanda endotoxin ndi kapangidwe kake kake.Machubuwa amapangidwa kuchokera kugalasi lapamwamba la borosilicate, lomwe limadziwika kuti limalimbana ndi dzimbiri lamankhwala.Izi zimawapangitsa kukhala abwino kuti agwiritsidwe ntchito poyezetsa endotoxin, chifukwa amatha kupirira kukhudzana ndi mitundu yambiri yoyesera popanda kuwononga kapena kuipitsa chitsanzo.

Chinthu china chofunikira cha machubu agalasi opanda endotoxin ndi ukhondo wawo.Machubuwa amatsukidwa bwino ndi kutsekeredwa musanagwiritse ntchito kuti achotse zomwe zingatengere matenda.Amayesedwanso mwamphamvu kuti atengeke ndi endotoxin, kuwonetsetsa kuti alibe kuchuluka kwazinthu zovulazazi.

Kuphatikiza apo, machubu agalasi opanda endotoxin adapangidwa kuti azikhala osavuta kugwiritsa ntchito.Amapezeka m'miyeso ndi masinthidwe osiyanasiyana kuti agwirizane ndi ma voliyumu osiyanasiyana a zitsanzo ndi njira zoyesera zonse zoyeserera zamtundu wa endotoxin komanso kuchuluka kwa mayeso a endotoxin.Zimagwirizananso ndi zida zosiyanasiyana zokonzekera ndi kuyesa zida, zomwe zimawapangitsa kukhala osinthika komanso osavuta pama labotale oyesa endotoxin.

Ponseponse, machubu agalasi opanda endotoxin amatenga gawo lofunikira pakulondola komanso kudalirika kwa kuyesa kwa endotoxin.Kupanga kwawo kwapamwamba kwambiri, kuyera, komanso kugwiritsa ntchito mosavuta kumawapangitsa kukhala gawo lofunikira pakuyesa kulikonse kopambana kwa endotoxin.

Machubu agalasi a Bioendo Endotoxin okhala ndi kukula kwake10 * 75mm, 12*75mm, 13*100mm ndi 16*100mmkwa dilutions ndondomeko ndi njira reaction.

Machubu agalasi opanda endotoxin amakwaniritsa mulingo wapamwamba kwambiri wa ma endotoxins ochepera 0.005EU/ml.

Machubu agalasi opanda endotoxin ayenera kugwiritsidwa ntchito poyesa kuyesa kwa gel clot endotoxin kuti mupewe zotsatira zabodza.
Endotoxins ndi ma cell a bakiteriya omwe amatha kuyipitsa zida za labotale, kuphatikiza machubu agalasi.
Kuyesa kwa gel clot endotoxin kumagwiritsidwa ntchito kuti azindikire kupezeka kwa endotoxins mu zitsanzo.Pakuyesa uku, magazi amapangidwa pamaso pa endotoxins.Kupanga kwa magazi kumeneku kumafaniziridwa ndi kuwongolera kuti mudziwe kuchuluka kwa endotoxin.
Kugwiritsa ntchito machubu agalasi opanda endotoxin kumathandiza kuwonetsetsa kuti kudziwika kwa endotoxin ndikolondola.Izi ndichifukwa choti ma endotoxins amatha kumamatira pamwamba pa machubu agalasi ndikusokoneza zotsatira zake.
Kuonetsetsa kuti machubu agalasi omwe amagwiritsidwa ntchito poyesa kuyesa kwa gel clot endotoxin alibe endotoxin, ayenera kutsukidwa ndi chotsukira kenako ndikuchapidwa bwino ndi madzi opanda endotoxin.Kuphatikiza apo, amayenera kutsukidwa pogwiritsa ntchito autoclaving kapena kutentha kowuma.
Pomaliza, kugwiritsa ntchito machubu agalasi opanda endotoxin mu kuyesa kwa gel clot endotoxin ndikofunikira kuti muwonetsetse kuti ma endotoxins apezeka molondola.Machubuwa amayenera kutsukidwa bwino ndi kuthiriridwa kuti athetse vuto lililonse.