Zinthu zopanda pyrogen zimatha kudyedwa popanda endotoxin yakunja, kuphatikiza nsonga za pipette zopanda pyrogen (bokosi la nsonga), machubu oyesa opanda pyrogen kapena machubu agalasi opanda endotoxin, ma ampoule agalasi opanda pyrogen, ma microplates opanda endotoxin opanda 96, ndi endotoxin- madzi aulere (kugwiritsira ntchito madzi a depyrogenated mu kuyesa kwa bakiteriya endotoxin), endotoxin free buffer ndi etc. Pakati pawo, madzi a bakiteriya endotoxintest ndi njira ya gel clot ndi kuchuluka kwa endotoxin test assay mu zolemba zonse za Pharmacopoeia (USP, EP, BP, JP) ndi China Pharmaceutica).Amatanthauza madzi osabala a jakisoni okhala ndi endotoxin yochepera 0.015EU/ml.Tsopano mtundu waposachedwa wa Pharmacopoeia, madzi a BET ndi ochepera 0.005EU/ml.Ngakhale muyezo wapamwamba kwambiri ndi wochepera 0.001EU/ml amatha kupangidwa ndikuperekedwa ndi Bioendo.
Mawu ofunikira okhudzana ndi endotoxins, ndi endotoxins free consumables, pyrogen ndi kutentha magwero ndi mfundo ziwiri zosiyana kotheratu:Purojeni: Amatchedwanso pyrogen kapena exothermic factor.zinthu zomwe zingayambitse kutentha kwa thupi.Gwero la Kutentha: Chinthu chomwe chimatulutsa kutentha.Monga machesi, makala, etc.Zomwe zimatchedwa "non-pyrogenic consumables" ndi "pyrogenic response" za opanga ena ndi amalonda kwenikweni ndi mayina osadziwika bwino komanso osocheretsa.Zolondola ziyenera kukhala "Pyrogen Free" ndi "Pyrogen Response".
Chifukwa chiyani zopangira zaulere za Pyrogen ndizofunikira pakuyesa kwa endotoxin, kuyesa kwa gel clot endotoxin test assay komanso kuchuluka kwa endotoxin test assay?
Inde, zogwiritsira ntchito zopanda pyrogen ndizofunikira pakuyesa kuyesa kwa endotoxin molondola komanso modalirika.Kukhalapo kwa ma pyrogens, omwe ndi zinthu zomwe zimayambitsa kutentha kwa thupi zomwe nthawi zambiri zimachokera ku mabakiteriya otchedwa endotoxins, zimatha kusokoneza zotsatira za mayesero ndikutsogolera kuwerengera zabodza.Mayeso a endotoxin, omwe amadziwika kuti Limulus amebocyte lysate (LAL) kapena otchedwa Lyophilized amebocyte lysate (LAL), amagwiritsidwa ntchito kuti azindikire ndikuwunika kupezeka kwa ma endotoxins a bakiteriya m'mankhwala, zida zamankhwala, ndi zinthu zina.Mayeso a LAL amadalira zomwe zimachitika pakati pa LAL reagent ndi endotoxins kuti apange kutsekeka kapena kuyankha kwa chromogenic.Kuti muwonetsetse zotsatira zolondola, ndikofunikira kugwiritsa ntchito zinthu zomwe zilibe ma pyrogens.Ma pyrogens amatha kuyipitsa zinthu zosiyanasiyana za labotale, kuphatikizamagalasi, nsonga za pipette, machubu, ndi zotengera zitsanzo.Ngati zinthu zomwe zili ndi pyrogen zitha kukhudzana ndi reagent ya LAL kapena zitsanzo zoyesa, zimatha kuyambitsa zinthu zabodza, zomwe zimabweretsa malingaliro olakwika okhudzana ndi kupezeka kapena kuchuluka kwa endotoxins.Zopangira zopanda pyrogen zimapangidwa ndikuyesedwa makamaka kuti zichepetse kapena kuthetsa kupezeka kwa ma pyrogen.Amakhala ndi njira zowongolera bwino kuti awonetsetse kuti akukwaniritsa zofunikira pakuyezetsa endotoxin.Kugwiritsa ntchito zida zapaderazi kumathandizira kusunga umphumphu ndi kulondola kwa kuyesa kwa endotoxin, kuwonetsetsa kuti zotsatira zodalirika komanso kulimbikitsa chitetezo cha odwala pazamankhwala ndi zamankhwala.
Nthawi yotumiza: Nov-07-2022
